Categories
Botany

Please write 2 pages about Codonopsis pilosula 1.what is the name of the plants

Please write 2 pages about Codonopsis pilosula
1.what is the name of the plants and the family
2.where this plant is cultivated and available
3.what are the chemical compounds available in these plant
4.which part of this plant is used traditionally
5.what is the traditional use of these plants
6. what is the antibacterial activity of this plant
7.what is the cytotoxic activity of this plant
8.what is the anti-inflammatory and antioxidant activity of this plant
9.what is the antibacterial activity of these compounds
Catalpol >98%
Aucubin >98%
Ajugol >98%
Jionoside B1 >98%
Melittoside >98%
Verbascoside >98%
Rehmannioside D >95%

Categories
Botany

Explain.

Writing a lab report between 9-10 pages.
Genetic Regulation
purpose: determine what mutations have occurred
in the different strains of E.coli. (012, ML 308-225,
W575)
β-galactosidase assay will determine which two
strains are mutated and which is the wild type
Genetic Regulation – procedure
Mix to resuspend any sediment in your samples.
add 10 drops of toluene in the fume hood. Mix and
incubate 15 min at 37oC.
Add 1mL 1% ONPG to each tube. Mix and incubate
15 min at 37oC.
◦ONPG: ortho-nitrophenyl-β-galactopyranoside
◦Cleaved by β-galactosidase – yields yellow
compound (ONP)
Genetic Regulation – procedure cont’d
Add 2 mL of 2% K2CO3 (stops reaction). Keep
samples in the hood.
Judge the degree of yellow color compared to
the control.
◦– (no color )
◦+ ,++,+++,++++ (increasing yellow color
intensity)
◦ Report results to TA for class data
Conjugation
We will plate out the cells to determine if either
of the plasmids (containing gldA or gldB)
restore motility to our gliding motility mutant
Procedure
◦ Scrape cells off mating plate with sterile applicator and
box streak* (supplement p. 44) on a PY2 plate with
erythromycin and a PY2 plate without antibiotic
◦ Do this for each conjugation plate, use a new applicator
for each streak
◦ Incubate at room temperature in a sealed container
At the end of the report answer these questions:
Questions for lab report:

1. Do the results show that expression of -galactosidase is inducible? If so what is
the inducer?

2. Is catabolite repression demonstrated by any of the cultures?

3. One of the strains used was wild type, and the other two were mutants. Based on
your observation of the phenotypes of each strain, and your knowledge of the lac
operon, which two are likely to be the mutants? What gene or region is likely to be
mutated in each of these two strains? Explain.

Categories
Botany

Explain.

Writing a lab report between 9-10 pages.
Genetic Regulation
purpose: determine what mutations have occurred in the different strains of E.coli. (012, ML 308-225, W575)
β-galactosidase assay will determine which two strains are mutated and which is the wild type
Genetic Regulation – procedure
Mix to resuspend any sediment in your samples.
add 10 drops of toluene in the fume hood. Mix and incubate 15 min at 37oC.
Add 1mL 1% ONPG to each tube. Mix and incubate 15 min at 37oC. ◦ONPG: ortho-nitrophenyl-β-galactopyranoside
◦Cleaved by β-galactosidase – yields yellow compound (ONP)
Genetic Regulation – procedure cont’d
Add 2 mL of 2% K2CO3 (stops reaction). Keep samples in the hood.
Judge the degree of yellow color compared to the control. ◦– (no color )
◦+ ,++,+++,++++ (increasing yellow color intensity)
◦ Report results to TA for class data
Conjugation
We will plate out the cells to determine if either of the plasmids (containing gldA or gldB) restore motility to our gliding motility mutant
Procedure
◦ Scrape cells off mating plate with sterile applicator and box streak* (supplement p. 44) on a PY2 plate with erythromycin and a PY2 plate without antibiotic
◦ Do this for each conjugation plate, use a new applicator for each streak
◦ Incubate at room temperature in a sealed container
At the end of the report answer these questions:
Questions for lab report: 1. Do the results show that expression of -galactosidase is inducible? If so what is the inducer? 2. Is catabolite repression demonstrated by any of the cultures? 3. One of the strains used was wild type, and the other two were mutants. Based on your observation of the phenotypes of each strain, and your knowledge of the lac operon, which two are likely to be the mutants? What gene or region is likely to be mutated in each of these two strains? Explain.

Categories
Botany

Keep your answers simple and easy to understand and there is no need to go more than a few sentences/a paragraph for each question.

Attached is the assignment consisting of a total of 4 questions. Keep your answers simple and easy to understand and there is no need to go more than a few sentences/a paragraph for each question. Thanks!

Categories
Botany

In order to figure out which species is most closely related to which and the order of divergence events, do you want to use a gene that evolves very quickly and will have a lot of changes in the region of dna you are comparing, or do you want to work with a gene that evolves very slowly and will have few to no differences in the dna between lineages?

Question 1: Name two specific proteins involved in basic cell function of all plants (or all eukaryotes) that you would expect to be present in all plants and have highly conserved sequence.
Question 2: When selecting genes to build a molecular phylogeny, one must consider how long ago the species involved diverged from one another. Imagine you are trying to determine relationships between a number of Drosophila species that you know to all have diverged (relatively) very recently, within the last 5-15 million years. In order to figure out which species is most closely related to which and the order of divergence events, do you want to use a gene that evolves very quickly and will have a lot of changes in the region of DNA you are comparing, or do you want to work with a gene that evolves very slowly and will have few to no differences in the DNA between lineages? Explain your answer.